In the face of growing requirements to reduce exhaust emissions and the need for more ecological solutions in the transportation sector, increasing attention is being paid to alternative fuels. This article examines the potential of using fuel mixtures containing bio-components, in particular alcohols and Sustainable Aviation Fuel (SAF), as a substitute for conventional fuels. The research focuses on two key aspects: composition of the fuel mixture and exhaust emissions. The research results aim to determine whether the proposed alternative fuel mixture can be an effective and more environmentally friendly alternative to conventional fuels, while maintaining suitable operating properties. The results of this work can be a valuable contribution to the development of sustainable fuel technologies, helping to reduce the negative impact of transportation on the environment.